Simply enter the DNA variant you would like to analyse into the Variant (VCF) field,
select one or multiple transcription factors and click on Submit. If you do not
know the location but have a wild-type and a variant sequence, you can still enter them.
In this case, ePOSSUM2 cannot display any known transcription binding sites.
ePOSSUM2 currently only works with human transcription factors.
ePOSSUM2 supports more than 1,000 different binding models for 247 human transcription
factors. The models were pooled from various publicly accessible data sources, such as
103 transcription factor flexible models (TFFMs) are included.
For each binding model, ePOSSUM2 constructs a Bayes classifier and reports the likelihood that a variant
leads to the gain or loss of a transcription factor binding site. Because of the poor performance of
several binding models in detecting experimentally verified sites, a negative or positive predictive
value is reported.